Search Search


Maskarinec SA, Franck C, Tirrell DA, Ravichandran G.
Quantifying cellular traction forces in three dimensions. 
Proc Natl Acad Sci U S A. 2009;106(52):22108-13.
PMID: 20018765

Schmitt E, Tanrikulu IC, Yoo TH, Panvert M, Tirrell DA, Mechulam Y., 
Switching from an induced-fit to a lock-and-key mechanism in an aminoacyl-tRNA synthetase with modified specificity.
J Mol Biol. 2009;394(5):843-51. 
PMID: 19837083

Zhang K, Sugawara A, Tirrell DA. 
Generation of surface-bound multicomponent protein gradients. Chembiochem. 2009 ;10(16):2617-9.
PMID: 19780079

Tanrikulu IC, Schmitt E, Mechulam Y, Goddard WA 3rd, Tirrell DA.
Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo.
Proc Natl Acad Sci U S A. 2009.
PMID: 19706454

Ngo JT, Champion JA, Mahdavi A, Tanrikulu IC, Beatty KE, Connor RE, Yoo TH, Dieterich DC, Schuman EM, Tirrell DA.
Cell-selective metabolic labeling of proteins. 
Nat Chem Biol. 2009.
PMID: 19668194

Tang Y, Wang P, Van Deventer JA, Link AJ, Tirrell DA.
Introduction of an Aliphatic Ketone into Recombinant Proteins in a Bacterial Strain that Overexpresses an Editing-Impaired Leucyl-tRNA Synthetase.Chembiochem. 2009.
PMID: 19670197

Montclare JK, Son S, Clark GA, Kumar K, Tirrell DA.
Biosynthesis and stability of coiled-coil peptides containing (2S,4R)-5,5,5-trifluoroleucine and (2S,4S)-5,5,5-trifluoroleucine.
Chembiochem. 2009;10(1):84-6.
PMID: 19090517

Liu JC, Tirrell DA.
Cell Response to RGD Density in Cross-Linked Artificial Extracellular Matrix Protein Films.
Biomacromolecules. 2008. 
PMID: 18826275 

Beatty KE, Tirrell DA.
Two-color labeling of temporally defined protein populations in mammalian cells.
Bioorg Med Chem Lett. 2008.
PMID: 18774715

Strable E, Prasuhn DE Jr, Udit AK, Brown S, Link AJ, Ngo JT, Lander G, Quispe J, Potter CS, Carragher B, Tirrell DA, Finn MG.
Unnatural amino acid incorporation into virus-like particles.
Bioconjug Chem. 2008;19(4):866-75. 
PMID: 18318461 

Connor RE, Piatkov K, Varshavsky A, Tirrell DA.
Enzymatic N-terminal addition of noncanonical amino acids to peptides and proteins.
Chembiochem. 2008;9(3):366-9. 
PMID: 18203209 

Wang A, Winblade Nairn N, Johnson RS, Tirrell DA, Grabstein K.
Processing of N-terminal unnatural amino acids in recombinant human interferon-beta in Escherichia coli.
Chembiochem. 2008;9(2):324-30.
PMID: 18098265 

Yoo TH, Link AJ, Tirrell DA.
Evolution of a fluorinated green fluorescent protein.
Proc Natl Acad Sci U S A. 2007;104(35):13887-90. 
PMID: 17717085 

Link AJ, Vink MK, Tirrell DA.
Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor.
Nat Protoc. 2007;2(8):1884-7.
PMID: 17703199 

Link AJ, Vink MK, Tirrell DA.
Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer.
Nat Protoc. 2007;2(8):1879-83.
PMID: 17703198 

Kwon I, Tirrell DA.
Site-specific incorporation of tryptophan analogues into recombinant proteins in bacterial cells.
J Am Chem Soc. 2007;129(34):10431-7. Epub 2007 Aug 8.
PMID: 17685515 

Yoo TH, Tirrell DA.
High-throughput screening for methionyl-tRNA synthetases that enable residue-specific incorporation of noncanonical amino acids into recombinant proteins in bacterial cells.
Angew Chem Int Ed Engl. 2007;46(28):5340-3. 
PMID: 17568466 

Dieterich DC, Lee JJ, Link AJ, Graumann J, Tirrell DA, Schuman EM.
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.
Nat Protoc. 2007;2(3):532-40.
PMID: 17406607 

Carrico IS, Maskarinec SA, Heilshorn SC, Mock ML, Liu JC, Nowatzki PJ, Franck C, Ravichandran G, Tirrell DA.
Lithographic patterning of photoreactive cell-adhesive proteins.
J Am Chem Soc. 2007;129(16):4874-5. Epub 2007 Mar 31. 
PMID: 17397163 

Beatty KE, Liu JC, Xie F, Dieterich DC, Schuman EM, Wang Q, Tirrell DA.
Fluorescence visualization of newly synthesized proteins in mammalian cells.
Angew Chem Int Ed Engl. 2006;45(44):7364-7. 
PMID: 17036290 

Kwon I, Wang P, Tirrell DA.
Design of a bacterial host for site-specific incorporation of p-bromophenylalanine into recombinant proteins.
J Am Chem Soc. 2006;128(36):11778-83.
PMID: 16953616 

Link AJ, Vink MK, Agard NJ, Prescher JA, Bertozzi CR, Tirrell DA.
Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids.
Proc Natl Acad Sci U S A. 2006;103(27):10180-5. Epub 2006 Jun 26.
PMID: 16801548 

Dieterich DC, Link AJ, Graumann J, Tirrell DA, Schuman EM.
Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
Proc Natl Acad Sci U S A. 2006;103(25):9482-7. Epub 2006 Jun 12.
PMID: 16769897

Montclare JK, Tirrell DA.
Evolving proteins of novel composition.
Angew Chem Int Ed Engl. 2006;45(27):4518-21. 
PMID: 16763955 

Son S, Tanrikulu IC, Tirrell DA.
Stabilization of bzip peptides through incorporation of fluorinated aliphatic residues.
Chembiochem. 2006;7(8):1251-7.
PMID: 16758500 

Diehl MR, Zhang K, Lee HJ, Tirrell DA.
Engineering cooperativity in biomotor-protein assemblies.
Science. 2006;311(5766):1468-71.
PMID: 16527982 

Shen W, Zhang K, Kornfield JA, Tirrell DA.
Tuning the erosion rate of artificial protein hydrogels through control of network topology.
Nat Mater. 2006;5(2):153-8. Epub 2006 Jan 29.
PMID: 16444261 

Mock ML, Michon T, van Hest JC, Tirrell DA.
Stereoselective incorporation of an unsaturated isoleucine analogue into a protein expressed in E. coli.
Chembiochem. 2006;7(1):83-7.
PMID: 16397872 

Link AJ, Tirrell DA.
Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition.
J Am Chem Soc. 2003;125(37):11164-5.
PMID: 16220915 

Beatty KE, Xie F, Wang Q, Tirrell DA.
Selective dye-labeling of newly synthesized proteins in bacterial cells.
J Am Chem Soc. 2005 ;127(41):14150-1.
PMID: 16218586 

Cantrill SJ, Grubbs RH, Lanari D, Leung KC, Nelson A, Poulin-Kerstien KG, Smidt SP, Stoddart JF, Tirrell DA.
Template-directed olefin cross metathesis.
Org Lett. 2005;7(19):4213-6.
PMID: 16146390 

Link AJ, Tirrell DA.
Reassignment of sense codons in vivo.
Methods. 2005;36(3):291-8.
PMID: 16076455 

Zhang K, Diehl MR, Tirrell DA.
Artificial polypeptide scaffold for protein immobilization.
J Am Chem Soc. 2005;127(29):10136-7.
PMID: 16028902 

Maskarinec SA, Tirrell DA.
Protein engineering approaches to biomaterials design.
Curr Opin Biotechnol. 2005;16(4):422-6. Review.
PMID: 16006115 

Stevens MM, Allen S, Davies MC, Roberts CJ, Sakata JK, Tendler SJ, Tirrell DA, Williams PM.
Molecular level investigations of the inter- and intramolecular interactions of pH-responsive artificial triblock proteins.
Biomacromolecules. 2005;6(3):1266-71.
PMID: 15877341 

Chen T, McIntosh D, He Y, Kim J, Tirrell DA, Scherrer P, Fenske DB, Sandhu AP, Cullis PR.
Alkylated derivatives of poly(ethylacrylic acid) can be inserted into preformed liposomes and trigger pH-dependent intracellular delivery of liposomal contents.
Mol Membr Biol. 2004;21(6):385-93.
PMID: 15764368 

Smeenk JM, Otten MB, Thies J, Tirrell DA, Stunnenberg HG, van Hest JC.
Controlled assembly of macromolecular beta-sheet fibrils.
Angew Chem Int Ed Engl. 2005;44(13):1968-71. 
PMID: 15724260 

Heilshorn SC, Liu JC, Tirrell DA.
Cell-binding domain context affects cell behavior on engineered proteins.
Biomacromolecules. 2005;6(1):318-23.
PMID: 15638535 

Richman GP, Tirrell DA, Asthagiri AR.
Quantitatively distinct requirements for signaling-competent cell spreading on engineered versus natural adhesion ligands.
J Control Release. 2005;101(1-3):3-12.
PMID: 15588889 

Link AJ, Vink MK, Tirrell DA.
Presentation and detection of azide functionality in bacterial cell surface proteins.
J Am Chem Soc. 2004;126(34):10598-602.
PMID: 15327317 

Stevens MM, Allen S, Sakata JK, Davies MC, Roberts CJ, Tendler SJ, Tirrell DA, Williams PM.
pH-dependent behavior of surface-immobilized artificial leucine zipper proteins.
Langmuir. 2004;20(18):7747-52.
PMID: 15323527 

Tirrell DA.
Chemical biology: hitting the sweet spot.
Nature. 2004;430(7002):837. 
PMID: 15318200 

Wang P, Fichera A, Kumar K, Tirrell DA.
Alternative translations of a single RNA message: an identity switch of (2S,3R)-4,4,4-trifluorovaline between valine and isoleucine codons.
Angew Chem Int Ed Engl. 2004;43(28):3664-6. 
PMID: 15248267 

Sneer R, Weygand MJ, Kjaer K, Tirrell DA, Rapaport H.
Parallel beta-sheet assemblies at interfaces.
Chemphyschem. 2004;5(5):747-50. 
PMID: 15179733 

Langer R, Tirrell DA.
Designing materials for biology and medicine.
Nature. 2004;428(6982):487-92. Review.
PMID: 15057821 

Liu JC, Heilshorn SC, Tirrell DA.
Comparative cell response to artificial extracellular matrix proteins containing the RGD and CS5 cell-binding domains.
Biomacromolecules. 2004;5(2):497-504.
PMID: 15003012 

Liu CY, Westerlund U, Svensson M, Moe MC, Varghese M, Berg-Johnsen J, Apuzzo ML, Tirrell DA, Langmoen IA.
Artificial niches for human adult neural stem cells: possibility for autologous transplantation therapy.
J Hematother Stem Cell Res. 2003;12(6):689-99. Review.
PMID: 14977478 

Link AJ, Mock ML, Tirrell DA.
Non-canonical amino acids in protein engineering.
Curr Opin Biotechnol. 2003;14(6):603-9. Review.
PMID: 14662389 

Nowatzki PJ, Tirrell DA.
Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking.
Biomaterials. 2004;25(7-8):1261-7.
PMID: 14643600 

Yamaoka T, Tamura T, Seto Y, Tada T, Kunugi S, Tirrell DA.
Mechanism for the phase transition of a genetically engineered elastin model peptide (VPGIG)40 in aqueous solution.
Biomacromolecules. 2003;4(6):1680-5.
PMID: 14606895 

Cirino PC, Tang Y, Takahashi K, Tirrell DA, Arnold FH.
Global incorporation of norleucine in place of methionine in cytochrome P450 BM-3 heme domain increases peroxygenase activity.
Biotechnol Bioeng. 2003;83(6):729-34.
PMID: 12889037 

Heilshorn SC, DiZio KA, Welsh ER, Tirrell DA.
Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins.
Biomaterials. 2003;24(23):4245-52.
PMID: 12853256 

Kwon I, Kirshenbaum K, Tirrell DA.
Breaking the degeneracy of the genetic code.
J Am Chem Soc. 2003;125(25):7512-3.
PMID: 12812480 

Wang P, Tang Y, Tirrell DA.
Incorporation of trifluoroisoleucine into proteins in vivo.
J Am Chem Soc. 2003;125(23):6900-6.
PMID: 12783542 

Liu CY, Apuzzo ML, Tirrell DA.
Engineering of the extracellular matrix: working toward neural stem cell programming and neurorestoration--concept and progress report.
Neurosurgery. 2003;52(5):1154-65; discussion 1165-7. Review.
PMID: 12699561 

Wang P, Vaidehi N, Tirrell DA, Goddard WA 3rd.
Virtual screening for binding of phenylalanine analogues to phenylalanyl-tRNA synthetase.
J Am Chem Soc. 2002;124(48):14442-9.
PMID: 12452720 

Tang Y, Tirrell DA.
Attenuation of the editing activity of the Escherichia coli leucyl-tRNA synthetase allows incorporation of novel amino acids into proteins in vivo.
Biochemistry. 2002;41(34):10635-45.
PMID: 12186549 

Rapaport H, Möller G, Knobler CM, Jensen TR, Kjaer K, Leiserowitz L, Tirrell DA.
Assembly of triple-stranded beta-sheet peptides at interfaces.
J Am Chem Soc. 2002;124(32):9342-3.
PMID: 12167007 

Datta D, Wang P, Carrico IS, Mayo SL, Tirrell DA.
A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins.
J Am Chem Soc. 2002;124(20):5652-3.
PMID: 12010034 

Kirshenbaum K, Carrico IS, Tirrell DA.
Biosynthesis of proteins incorporating a versatile set of phenylalanine analogues.
Chembiochem. 2002;3(2-3):235-7. 
PMID: 11921403 

Kiick KL, Saxon E, Tirrell DA, Bertozzi CR.
Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation.
Proc Natl Acad Sci U S A. 2002;99(1):19-24. 
PMID: 11752401 

Mawn MV, Fournier MJ, Tirrell DA, Mason TL.
Depletion of free 30S ribosomal subunits in Escherichia coli by expression of RNA containing Shine-Dalgarno-like sequences.
J Bacteriol. 2002;184(2):494-502.
PMID: 11751827

Tang Y, Ghirlanda G, Petka WA, Nakajima T, DeGrado WF, Tirrell DA.
Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability 
Angew Chem Int Ed Engl. 2001;40(8):1494-1496.
PMID: 11317312

Kiick KL, Tirrell DA.
Protein Engineering by In Vivo Incorporation of Non-natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase.
Tetrahedron 56: 9487-9493 (2000).  

Tamura T, Yamaoka T, Kunugi S, Panitch A, Tirrell DA.
Effects of Temperature and Pressure on the Aggregation Properties of an Engineered Elastin Model Polypeptide in Aqueous Solution.
Biomacromolecules 1:  552-555 (2000).  

Yu SM, Tirrell DA.
Thermal and Structural Properties of Biologically Derived Monodisperse Hairy-Rod Polymers.
Biomacromolecules 1: 310-312 (2000).  

Rapaport H, Kjaer K, Jensen TR, Leiserowitz L, Tirrell DA.
Two-Dimensional Order in ß-sheet Peptide Monolayers.
J. Am. Chem. Soc. 122: 12523-12529 (2000).  

Yu SM, Soto CM, Tirrell DA.
Nanometer-Scale Smectic Ordering of Genetically Engineered Rod-Like Polymers:  Synthesis and Characterization of Monodisperse Derivatives of Poly(g-benzyl a,L-glutamate).
J. Am. Chem. Soc. 122: 6552-6559 (2000).  

Thomas JL, Tirrell DA.
Polymer-Induced Leakage of Cations from Dioleoyl Phosphatidylcholine and Phosphatidylglycerol Liposomes.
J. Control. Release 67: 203-209 (2000).  

Kiick KL, van Hest JCM, Tirrell DA.
Expanding the Scope of Protein Biosynthesis by Altering the Methionyl-tRNA Synthetase Activity of a Bacterial Expression Host.
Angew. Chem. Int. Ed. 39: 2148-2152 (2000).  

Welsh ER, Tirrell DA.
Engineering the Extracellular Matrix:  A Novel Approach to Polymeric Biomaterials. I. Control of Physical Properties of Artificial Protein Matrices Designed to Support Adhesion of Vascular Endothelial Cells.
Biomacromolecules 1: 23-30 (2000).  

Michon T, Tirrell DA.
Des Protéines Artificielles Pour de Nouveaux Matériaux.
Biofutur 197: 34-38 (2000).  

Linhardt JG, Tirrell DA.
Utilizing the Hydrophobic Polyelectrolyte, Poly(2-Ethylacrylic Acid), for Drug Delivery.
ACS Symp. Ser. 752: 243 (2000).

Sharma N, Furter R, Kast P, Tirrell DA.
Efficient Introduction of Aryl Bromide Functionality into Proteins in vivo.
FEBS Lett. 467: 37-40 (2000).  

van Hest JCM, Kiick KL, Tirrell DA.
Efficient Incorporation of Unsaturated Methionine Analogues into Proteins in vivo.
J. Am. Chem. Soc. 122: 1282-1288 (2000).  

Linhardt JG, Tirrell DA.
pH-induced Fusion and Lysis of Phosphatidylcholine Vesicles by the Hydrophobic Polyelectrolyte, Poly (2-Ethylacrylic Acid).
Langmuir 16: 122-127 (2000).